A method for dextruxin analysis by HPLC-PDA-ELSD-MS

Analysis of polysorbate 80 and its related compounds by RP-HPLC with ELSD and MS detection.

The chemical composition of polysorbate 80 strongly influences the physicochemical properties and performance of many products. Consequently, a reliable characterization of polysorbate 80 is crucial for many applications. However, the exact composition of these chemical mixtures cannot be determined by colorimetry, hydrolysis, size-exclusion chromatography, nuclear magnetic resonance or mass sp...

Detection of isorhamnetin glycosides in extracts of apples (Malus domestica cv. "Brettacher") by HPLC-PDA and HPLC-APCI-MS/MS.

Extracts of apple fruits (Malus domestica cv. "Brettacher") were analysed by HPLC with photodiode array detection. An unknown peak was monitored displaying the same retention time as isorhamnetin 3-O-glucoside. Preliminary identification of the isorhamnetin aglycone was performed by comparison of UV spectral data of the unknown compound with a reference substance. Using atmospheric pressure che...

A New HPLC-PDA Method for the Analysis of Tyrian Purple Components

A common HPLC-PDA method for amino acid analysis in insects and plants.

A common method for analysis of 17 amino acids from various insect species and plant parts was standardized using HPLC-PDA. Prior to hydrolysis, lyophilization of test samples was found indispensible to remove excess moisture, which interferes in hydrolysis and separation of amino acids. After the hydrolysis of plant and insect samples, 500 and 100 microL of boiling HCl, respectively for recons...

Identification and Confirmatory Method for Human Chorionic Gonadotropin (hCG) using HPLC/MS/MS Analysis

Author for correspondence: Dr Gam Lay Harn School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia. Tel: 604-6533888 ext 2208 Fax: 604-6570017 E-mail: [email protected] Abstract As a consequence of the abused of human chorionic gonadotropin αhCG) by male athletes, a reliable detection method for βhCG is highly desired in sport testing. hCG is misused by m...